N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO
Guifang Jia,Ye Fu,Xu Zhao,Qing Dai,Guanqun Zheng,Ying Yang,Chengqi Yi,Tomas Lindahl,Tao Pan,Yun-Gui Yang, Chuan He Nature Chemical Biology. 2011,7,885–8870 Abstract： We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m6A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m6A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m6A in nuclear RNA is a major physiological substrate of FTO.