Yuan Wang, Hanfu Wei, Qin Pan, Zhaohui Wang, Rui Xing, Wenmei Li, Jun Zhang, Meng Ding, Jiao Guo, Lin Wu, Youyong Lu, Siqi Liu

Analytical Biochemistry

Abstract

High background interference during the antibody pair screening process is inevitable. In this study, we found that the high background was associated with heterophilic antibody interference introduced by the application of ascites-derived monoclonal antibodies when conducting large-scale antibody pair screening against different proteins. To eliminate antibody-associated heterophilic antibody interference, both blocking with mouse normal sera and antigen-mediated affinity chromatography were used, resulting in significant improvement in pairing performance and in antibody pair screening efficiency.