Recently, a brief introduction of research strategies of bacterial genome and transcriptome based on the next-generation sequencing technologies was released by Professor HU Songnian’s group, Beijing Institute of Genomics (BIG), Chinese Academy of Sciences (CAS). Since many bacterial genome and transcriptome studies have been performed based on the next-generation sequencing technologies, opportunities come along with challenges in these fields, thus how to select the appropriate research strategies is becoming increasingly important.

This paper reviewed conventional methods and procedures and presented the existing problems briefly for bacterial genome and transcriptome studies. The research strategies provide a relatively complete pipeline for the majority of bacteria, including sample collection, sequencing platform selection, data process, database and software applying and downstream biological study. Moreover, it will promote the research of other fields, such as the course of life, biological evolution, basal metabolism, disease and drugs.

The review was published in Microbiology China on Nov 20, 2011.

(The research strategies of bacteria genome and transcriptome based on the next-generation sequencing technologies, image by LIU Wanfei )

Paper link: http://journals.im.ac.cn/wswxtbcn/ch/reader/view_abstract.aspx?file_no=tb11111705&flag=1

Contact: Professor HU Songnian  Email: husn@big.ac.cn